黄东益
详细
作者： 杨亚飞，刘沈徽，黄东益，吴文嫱 （海南大学热带农林学院）
出处： 热带生物学报 2020
关键词： 大薯；嫩叶；染色体制备；荧光原位杂交
摘要： 为克服利用根尖制备染色体标本的局限性,拟通过优化酶解去壁低渗法中的材料幼嫩程度、预处理方法以及酶解时间,建立大薯嫩叶染色体制备技术体系。结果表明:取刚展开的大薯嫩叶,用0.002 mol·L -1 8-羟基喹啉于4℃下预处理2 h,用3.5%纤维素酶和1.75%的果胶酶混合溶液于37℃下解离1 h, ...
作者： 陈伟益，黄东益，吴文嫱，夏薇，黄小龙，许云，张荣萍 （海南大学热带作物学院）
出处： 海南大学学报(自然科学版) 2020 第2期 P141-146
关键词： 淮山；壳聚糖；炭疽病；连作
摘要： 本文针对连作淮山炭疽病难以防控的问题,采用离体菌落培养与大田栽培相结合的方式,在淮山长期连作、炭疽病严重的的地块上种植淮山,研究壳聚糖及其衍生物壳寡糖、禾甲安对病原菌菌落直径、抑菌持效期及大田炭疽病发病情况的影响.结果表明,壳聚糖及壳寡糖可以抑制病原菌菌丝伸长,改变菌丝结构,抑制效果最好的为壳寡糖1 ...
作者： 肖珂，周双清，许云，吴文嫱，夏薇，张荣萍，黄东益，黄小龙 （热带生物资源教育部重点实验室；海南大学热带作物学院；海南大学园艺学院）
出处： 热带生物学报 2020 第2期 P156-162
关键词： 海绵；放线菌；分离；抑菌活性
摘要： 为了探究海南海绵共附生放线菌资源的多样性及潜在的药用价值,采用4种分离培养基分离海南文昌海域海绵的共附生放线菌,利用16S rRNA序列分析对分离的放线菌进行鉴定,采用滤纸片扩散法对分离放线菌的发酵产物进行抗菌活性分析。结果表明,从4种海绵中共获得50株放线菌,所分离菌株归属于5个亚目、6个科和7个 ...
作者： 张金兰，张博，黄东益，肖勇，吴文嫱，许云，黄小龙，夏薇 （海南大学热带农林学院；中国热带农业科学院椰子研究所）
出处： 分子植物育种 2019 第17卷 第7期 P2086-2093
关键词： 大薯(Dioscorea；alata；L.)；ERF；基因家族
摘要： 大薯属于薯蓣科薯蓣属,是热带和亚热带地区的一种重要的粮食作物,但其基础研究还较为薄弱。本研究基于公布的大薯转录谱序列和基因组序列,开展了对大薯ERF家族基因的挖掘和特征分析。共找到DaERF基因41个,其中14个DaERF有一个以上的外显子,其余的均为一个外显子。进一步的blastx分析发现30个D ...
作者： 杨亚飞，黎丹，黄东益，符晓，夏薇，吴文嫱 （海南大学热带农林学院）
出处： 植物遗传资源学报 2019 第20卷 第4期 P1087-1092
关键词： 毛薯；硒代半胱氨酸甲基转移酶；基因克隆；生物信息学；表达分析
摘要： 硒代半胱氨酸甲基转移酶(SMT,Selenocysteine methyltransferase)是植物硒代谢的关键酶,在植物硒积累的过程中发挥着重要作用。为了探究毛薯中SMT基因的功能,本研究以优质富硒毛薯Ds148为研究对象,利用RT-PCR技术从中克隆到了毛薯SMT基因,命名为DsSMT。Ds ...
作者： Liu, JP (Liu, Jin-Ping);Hu, J (Hu, Jin);Liu, YH (Liu, Yan-Hui);Yang, CP (Yang, Cui-Ping);Zhuang, YF (Zhuang, Yu-Fen);Guo, XL (Guo, Xiu-Li);Li, YJ (Li, Yi-Jian);Zhang, LS (Zhang, Liangsheng) （;[ 1 ] Hainan Univ, Trop Agr & Forestry Inst, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Hainan, Peoples R China [ 2 ] Fujian Agr & Forestry Univ, Ctr Genom & Biotechnol, Key Lab Minist Educ Genet Breeding & Multiple Uti, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Fujian, Peoples R China [ 3 ] Chinese Acad Trop Agr Sci, Rubber Res Inst, Serv Ctr Sci & Technol, Danzhou 571737, Hainan, Peoples R China）
出处： Physiology and Molecular Biology of Plants 2018 Vol.24 No.3 P349-358
关键词： Methyl jasmonate;RNA sequencing;Rubber biosynthesis;Rubber tree;Transcriptome
摘要： The phytohomorne methyl jasmonate (MeJA) is known to trigger extensive reprogramming of gene expression leading to transcriptional activation of many ...
作者： Liu, F.;Lan, X.-T.;Hou, T.;Kang, B.;Liu, Y.;Lin, C.-X. （a State Key Laboratory of Automotive Simulation and Control, Jilin University, Changchun, 130022, China; b College of Communication Engineering, Jilin University, Changchun, 130022, China; c College of Information Science &Technology, Hainan University, Haikou, 570228, China）
出处： Jilin Daxue Xuebao (Gongxueban)/Journal of Jilin University (Engineering and Technology Edition) 2018 Vol.48 No.5 P1593-1599
关键词： Computer application;Fuzzy C-means method;K-mer;Metagenome;Non-negative matrix factorization(NMF);Pattern recognition and intelligent systems
摘要： DNA sequence classification is a very important step in metagenomic study, and k-mer frequency is a commonly used feature for DNA sequence classificat ...
作者： Liu, Y.;Xiao, H.;Yao, K.;Hu, J.;Wei, H. （a State Key Laboratory of Water Resources and Hydropower Engineering Science, Institute of Engineering Risk and Disaster Prevention, Wuhan University, Wuhan 430072, China; b Department of Civil & Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, Singapore, 117576, Singapore; c College of Civil Engineering and Architecture, Hainan University, 58 Renmin Road, Meilan District, Haikou, Hainan 570228, China）
出处： Geoscience Frontiers 2018 Vol.9 No.6 P1649-1655
关键词： Finite-element modelling;Monte-Carlo simulations;Numerical computation;Random fields;Slopes stability;Statistical analysis
摘要： To investigate the strong random nature of the geometric interfaces between soil and rock, a rock-soil slope is considered as a two-phase random mediu ...
作者： Liu, F.;Zong, Y.-X.;Kang, B.;Zhang, Y.-M.;Lin, C.-X.;Zhao, H.-W. （a National Key Laboratory for Automotive Simulation and Control, Jilin University, Changchun, 130022, China; b College of Communication Engineering, Jilin University, Changchun, 130022, China; c Electronic and Electrical Engineering Department, The University of Sheffield, Sheffield, S10 2TN, United Kingdom; d College of Information Science and Technology, Hainan University, Haikou, 570228, China; e College of Computer Science and Technology, Jilin University, Changchun, 130012, China）
出处： Jilin Daxue Xuebao (Gongxueban)/Journal of Jilin University (Engineering and Technology Edition) 2018 Vol.48 No.6 P1844-1850
关键词： Computer application;Dorsal hand vein recognition system;Texture feature optimization;Vein acquisition device
摘要： In order to extract and match the texture features of dorsal hand vein, this paper presents a dorsal hand vein recognition system based on optimized t ...
作者： Liu, Y.;Zhang, W.;Zhang, L.;Zhu, Z.;Hu, J.;Wei, H. （a State Key Laboratory of Water Resources and Hydropower Engineering Science, Institute of Engineering Risk and Disaster Prevention, Wuhan University, Wuhan, 430072, China; b School of Civil Engineering, Chongqing University, Chongqing, 400045, China; c School of Civil Engineering and Architecture, Wuhan University of Technology, Wuhan, 430070, China; d College of Civil Engineering, Hunan University, Changsha, 410012, China; e College of Civil Engineering and Architecture, Hainan University, Haikou, Hainan 570228, China）
出处： Geoscience Frontiers 2018 Vol.9 No.6 P1657-1664
关键词： Factor of safety;Finite-element modelling;Monte-Carlo simulations;Random field;Slope stability;Statistical analysis
摘要： A long slope consisting of spatially random soils is a common geographical feature. This paper examined the necessity of three-dimensional (3D) analys ...